Dr. Emily Roberts6 min read
What is HPLC Analysis?
High-Performance Liquid Chromatography (HPLC) is the gold standard for assessing peptide purity. This analytical technique separates, identifies, and quantifies each component in a mixture by passing it through a column under high pressure.
Why Purity Matters
For research applications, peptide purity directly impacts the reliability and reproducibility of experimental results. Impurities can include:
- Deletion sequences - peptides missing one or more amino acids
- Truncated sequences - incomplete synthesis products
- Oxidized forms - peptides with modified amino acids
- Salts and counterions - residual from synthesis or purification
Reading a Certificate of Analysis
Every batch from Miro Biotech includes a detailed Certificate of Analysis (COA). Key metrics to look for:
- Purity percentage - typically reported as area percentage from HPLC
- Retention time - confirms the identity of the peptide
- Mass spectrometry data - verifies the molecular weight
- Appearance - physical description of the lyophilized product
- Solubility - recommended solvents and expected behavior
Our Quality Commitment
At Miro Biotech, we maintain a minimum purity threshold of 98% for all products, with most exceeding 99%. Every batch undergoes independent third-party testing before release.